EXOSOME CRO · SERVICE PRICING
Lifeint’s official EV research service price list covers 84 services across eight modules: supernatant preparation, isolation, characterisation, QC, omics, differential validation, tracing and engineering, and functional studies. Services cover plant and animal samples and experience with 300+ plant species. Prices include tax and are indicative; the confirmed project order governs. Call +86 592 5792117 (WeChat +86 18965129960).
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Sample preparation Isolation and purification Characterization Quality control Omics analysis Validation Tracing & engineering Functional studies8 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Tissue enzymatic digestion (plant) | Use cellulase and pectinase digestion to prepare plant tissue supernatant for EV isolation. | 200CNY/sample | 3 business days | Tissue amount ≤3 g |
| Tissue enzymatic digestion (animal) | Prepare tissue supernatant using enzymes such as trypsin and collagenase for subsequent EV isolation. | 200CNY/sample | 3 business days | Tissue amount ≤3 g |
| Tissue culture (plate) | Mince tissue, pass through a cell strainer and culture on plates to prepare supernatant for subsequent EV isolation. | 10CNY/mL | 3 business days | Fresh tissue only; supernatant ≥20 mL per batch |
| Cell culture (2D) | Collect supernatant using standard adherent or suspension culture in dishes, flasks or cell factories. | 5CNY/mL | 10 business days | Special culture media require a separate quotation; supernatant ≥50 mL per batch |
| Cell culture (3D microcarriers) | Collect supernatant from 3D microcarrier suspension culture. | 2000CNY/L | 20 business days | Custom experiments require a separate quotation |
| Organoid culture (3D organoid) | Collect supernatant from 3D organoids established using matrix gel. | 4000CNY/batch | 20 business days | Custom experiments require a separate quotation |
| Microbial culture (fermentation) | Collect supernatant from bacterial or fungal fermentation using a temperature-controlled shaker. | 500CNY/L | 20 business days | Custom experiments require a separate quotation |
| Algal culture (illuminated culture) | Collect supernatant from microalgae cultured in an illuminated incubator or bioreactor. | 500CNY/L | 20 business days | Custom experiments require a separate quotation |
10 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Ultracentrifugation (classic workflow) Common | Isolate EVs using conventional differential ultracentrifugation (UC). | 200CNY/50 mL | 3 business days | Special samples requiring dilution, enzymatic digestion or non-standard handling require a separate quotation. |
| Ultracentrifugation (sucrose cushion) | Add a sucrose cushion to ultracentrifugation to further remove protein impurities and improve purity. | 300CNY/50 mL | 3 business days | — |
| Ultracentrifugation (iodixanol density gradient) | Isolate EVs using an iodixanol (OptiPrep) density gradient and ultracentrifugation. | 500CNY/50 mL | 3 business days | At least 6 samples per batch |
| Polymer precipitation (PEG) | EV isolation by PEG precipitation. | 200CNY/50 mL | 3 business days | — |
| Ultrasonic nanofiltration (EXODUS) | EV isolation using an ultrasound nanofiltration chip. | 600CNY/500 μL | 5 business days | Sample volume≤1ml |
| Large-volume sample isolation （EXO-DM） | Isolate EVs from large-volume samples using a combination of multistage membrane clarification, hollow-fibre or cassette tangential-flow concentration, and size-exclusion or ultracentrifugation purification. | 1500CNY/L | 10 business days | Sample volume≥3L |
| Magnetic-bead capture (membrane-sensing peptide) | Isolate total EVs using magnetic beads coupled to membrane-sensing peptides (MSPs). | 200CNY/sample | 3 business days | Sample volume≤1.5ml |
| Magnetic sorting (immunomagnetic beads) | Capture EVs expressing specific membrane proteins with antibody-coated magnetic beads, then sort and recover them using a magnetic rack. | 200CNY/sample | 3 business days | Antibodies and custom magnetic beads are not included. |
| Magnetic sorting (biotin beads) | Use bead-form polyacrylamide resin bearing immobilised recombinant streptavidin for biotin affinity purification, sorting and recovery of EVs expressing specific membrane proteins. | 400CNY/sample | 3 business days | Antibodies and sorting kits are not included. |
| Custom magnetic beads (subpopulation sorting) | Couple antibodies or peptides to magnetic beads for subsequent capture and sorting of specific EV subpopulations. | 3000CNY/batch | 7 business days | Antibodies or peptides are not included. |
7 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Transmission electron microscopy (TEM) Common | Observe negatively stained EV morphology by transmission electron microscopy. Six images are supplied by default: 1000 nm (1), 500 nm (1), 200 nm (1) and 100 nm (3). | 200CNY/sample | 3 business days | Custom imaging settings require a separate assessment. |
| Particle analysis （NanoFCM） Common | Measure particle size and concentration by NanoFCM. | 200CNY/sample | 3 business days | Recommended by default |
| Particle analysis （Zetaview） | Measure EV particle size and concentration by nanoparticle tracking analysis (ZetaView). | 200CNY/sample | 3 business days | — |
| Particle analysis （NS300） | Measure EV particle size and concentration by nanoparticle tracking analysis (NS300). | 400CNY/sample | 5 business days | — |
| Protein quantification （BCA） | Measure total protein concentration after EV lysis using the BCA assay. | 50CNY/sample | 1 business day | — |
| Protein analysis （Western Blot） Common | Assess EV-associated protein markers by Western blot. | 100CNY/sample/marker | 3 business days | A cell positive control and six antibody markers (CD9/CD63/CD81/TSG101/Syntenin1/Calnexin) are provided at no extra charge. Confirm antibody information for species other than human, rat or mouse. |
| Protein analysis （NanoFCM) Common | Assess EV-associated protein markers by nano-flow cytometry. | 200CNY/sample/marker | 3 business days | A negative control and three flow-cytometry antibody markers (CD9/CD63/CD81) are provided at no extra charge. Confirm antibody information for species other than human, rat or mouse. |
8 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Purity assessment (lysis method) | Measure the change in particle concentration before and after lysis with Triton-100 to assess EV purity. | 500CNY/sample | 3 business days | — |
| Purity assessment (dye method) | Label membranes with EVmembrane Green Stains and assess preparation quality using the assay readout. | 500CNY/sample | 3 business days | Applicable to animal-derived EVs only. |
| Zeta-potential measurement (ZetaView) | Measure EV zeta potential using the ZetaView nanoparticle analysis system. | 300CNY/sample | 3 business days | — |
| Osmolality measurement (SMC 30D) | Measure EV-preparation osmolality using the SMC 30D. | 200CNY/sample | 3 business days | — |
| Microbial testing (culture) | Add EVs to microbial culture medium and shake for 24 hours to test for bacterial or fungal contamination. | 200CNY/sample | 3 business days | — |
| Mycoplasma testing (qPCR) | Use qPCR to test EV preparations for residual mycoplasma DNA. | 200CNY/sample | 3 business days | — |
| Endotoxin testing (LAL assay) | Measure endotoxin using a limulus amebocyte lysate assay kit. | 200CNY/sample | 3 business days | — |
| Heavy-metal testing (atomic absorption) | Use atomic absorption spectroscopy (AAS) to measure heavy metals including lead, cadmium, chromium, mercury and arsenic in EV preparations. | 600CNY/sample | 5 business days | — |
12 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Nucleic acid extraction (RNA) | Extract RNA using a low-input EV RNA kit and quantify it by fluorometry. | 150CNY/sample | 2 business days | — |
| Nucleic acid extraction (DNA) | Extract DNA using a low-input EV DNA kit and quantify it by fluorometry. | 150CNY/sample | 2 business days | — |
| Nucleic-acid QC (capillary electrophoresis) | Assess EV nucleic-acid fragment distribution using capillary electrophoresis (Qsep100). | 50CNY/sample | 2 business days | — |
| Transcriptomics （miRNA） | Prepare and sequence miRNA libraries using QIAseq with standard analysis; output 10M reads (3G raw data), using Illumina by default. | 1000CNY/sample | 20 business days | Recommended RNA input: 10 ng; minimum library input: 1 ng. |
| Transcriptomics （LncRNA） | Prepare and sequence lncRNA libraries using Smart-seq with standard lncRNA and mRNA analysis; output 10G raw data, using Illumina by default. | 1000CNY/sample | 20 business days | Recommended RNA input: 10 ng; minimum library input: 1 ng. |
| Proteomics （DIA） | Perform DIA mass-spectrometry proteomics with standard analysis, using the Thermo Astral platform by default. | 1000CNY/sample | 20 business days | Recommended EV or protein input: 10 μg. |
| Metabolomics （LC-MS/MS） | Perform untargeted LC-MS/MS metabolomics, using the QE/HF-X platform by default. | 300CNY/sample | 20 business days | Recommended EV or protein input: 10 μg. |
| Plant metabolomics (LC-MS/MS) | Perform untargeted LC-MS/MS metabolomics for plant small molecules, including sugars, amino acids, nucleotides, alkaloids, flavonoids and phenolics. | 500CNY/sample | 20 business days | Recommended EV or protein input: 10 μg. |
| Lipidomics (LC-MS) | Perform relative-quantitative LC-MS/MS lipidomics, using the QE/HF-X platform by default. | 600CNY/sample | 20 business days | Recommended EV or protein input: 10 μg. |
| Microbiome analysis (Nanopore) | Sequence full-length 16S using the Nanopore long-read platform. | 500CNY/sample | 20 business days | Recommended input: 50 μg EVs or 20 ng DNA. |
| Genomics (WGS) | Prepare low-input DNA libraries for whole-genome sequencing and analysis. | 3000CNY/sample | 20 business days | Recommended input: 50 μg EVs or 20 ng DNA. |
| Membrane proteomics (PBA) | Measure surface proteins by single-EV proximity barcoding assay (PBA), using a 550-protein panel by default. | 7500CNY/sample | 30 business days | Recommended EV or protein input: 10 μg. |
7 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Gene validation （qPCR） | Quantify nucleic acids using real-time qPCR, with Bio-Rad CFX96 by default. | CNY 60/sample/gene | 3 business days | Includes reverse transcription and qPCR; primer synthesis is not included. |
| Gene validation （ddPCR） | Nucleic-acid quantification by digital PCR, using a Qiagen platform by default. | CNY 200/sample/gene | 7 business days | Probe- or dye-based assays; charges depend on requirements. |
| Gene validation (luciferase reporter) | Use a luciferase reporter to assess miRNA targeting of an mRNA 3′UTR or sponge lncRNA/circRNA, or transcription-factor regulation of a target gene. | 5000CNY/sample | 20 business days | Custom experiments require a separate quotation |
| Protein validation （Western Blot） | Protein-marker testing by Western blot. | CNY 800/membrane | 5 business days | Antibodies are charged separately. |
| Protein validation （ELISA） | Protein-marker testing by ELISA. | CNY 600/plate | 5 business days | Assay kits are charged separately. |
| Protein validation （CLIA） | Protein-marker testing by chemiluminescent immunoassay (CLIA). | CNY 600/plate | 5 business days | Assay kits are charged separately. |
| Protein validation （SPR） | Protein testing by surface plasmon resonance (SPR). | 250CNY/sample/Protein | 5 business days | Antibodies are charged separately. |
24 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Fluorescent labelling (dye) | Label EVs with fluorescent dyes such as PKH26 (red) and PKH67 (green). | 200CNY/sample | 3 business days | — |
| Fluorescent labelling (viral method) | Use lentivirus-mediated expression systems such as CD63-GFP for endogenous EV fluorescent labelling. | 3000CNY/sample | 20 business days | Custom experiments require a separate quotation |
| Tracing and imaging (cell confocal microscopy) | Co-incubate fluorescent EVs with cells and use confocal microscopy to observe uptake by recipient cells. | 500CNY/sample | 7 business days | Custom experiments require a separate quotation |
| Tracing and imaging (zebrafish fluorescence) | Add fluorescent EVs to zebrafish larval medium or inject them into zebrafish, then image by fluorescence stereomicroscopy. | CNY 5,000/10 animals | 20 business days | Custom experiments require a separate quotation |
| Tracing and imaging (small-animal in vivo imaging) | Inject fluorescent EVs into mice and image using a small-animal in vivo imaging system. | CNY 250/animal | 7 business days | Custom experiments require a separate quotation |
| Exogenous loading (focused ultrasound) | Use adaptive focused ultrasound to treat EVs and load small-molecule drugs. | 1000CNY/sample | 3 business days | Volume≤2ml |
| Exogenous loading (probe sonication) | Use probe sonication to treat EVs and load small-molecule drugs. | 500CNY/sample | 3 business days | — |
| Exogenous loading (transfection reagent) | Use cationic substances to promote interactions with lipid membranes and subsequent internalisation for nucleic-acid drug loading. | 1000CNY/sample | 3 business days | Transfection reagents are included. |
| Exogenous loading (lipid anchoring) | Insert DSPE-PEG into the EV phospholipid bilayer to anchor attached targeting or functional molecules to the EV surface. | 500CNY/sample | 3 business days | Peptide synthesis is not included. |
| Exogenous loading (affinity anchoring) | Modify EVs using CD63-binding peptides selected from phage libraries (such as CP05) to attach targeting or functional molecules to the surface. | 500CNY/sample | 3 business days | Peptide synthesis is not included. |
| Exogenous loading (click chemistry) | Attach targeting or functional molecules to the EV surface using click chemistry, such as SPAAC. | 1500CNY/sample | 10 business days | Peptide synthesis is not included. |
| Endogenous loading (gene knockdown) | Transfect plasmids or transduce parent cells to reduce target nucleic-acid or protein expression, affecting the target molecules in EVs. | 2000CNY/sample | 25 business days | Custom experiments require a separate quotation |
| Endogenous loading (gene overexpression) | Transfect plasmids or transduce parent cells to increase target nucleic-acid or protein expression, affecting the target molecules in EVs. | 4000CNY/sample | 25 business days | Custom experiments require a separate quotation |
| Biomimetic vesicles (cell vesiculation) | Extrude cells through micron-scale membranes to disrupt and reassemble membranes into nanovesicles of the desired size. | 1000CNY/sample | 5 business days | Custom experiments require a separate quotation |
| Hybrid vesicles (ultrasound + extrusion) | Treat two vesicle or liposome populations with low-frequency focused ultrasound, followed by extrusion to obtain fused vesicles of a specified size. | 1000CNY/sample | 5 business days | Custom experiments require a separate quotation |
| Cargo-loading assessment (qPCR) | Assess EV nucleic-acid loading by real-time quantitative PCR. | 600CNY/sample | 3 business days | Custom experiments require a separate quotation |
| Cargo-loading assessment (nano-flow cytometry) | Assess EV loading using nano-flow cytometry to detect drug-associated fluorescence. | 200CNY/sample | 3 business days | Custom experiments require a separate quotation |
| Cargo-loading assessment (HPLC) | Assess EV cargo loading by high-performance liquid chromatography (HPLC). | 1000CNY/sample | 10 business days | Custom experiments require a separate quotation |
| Cargo-loading assessment (LC-MS) | Assess EV cargo loading by liquid chromatography–mass spectrometry (LC-MS). | 1500CNY/sample | 10 business days | Custom experiments require a separate quotation |
| Cargo-loading assessment (derivatisation) | Assess EV cargo loading by quantification after addition of excess derivatising or fluorescent groups to produce UV absorption or fluorescence. | 1500CNY/sample | 5 business days | Custom experiments require a separate quotation |
| Lyophilisation (small scale) | Small lyophiliser capacity is approximately 200 mL per run, up to 100 vials at 2 mL each. | 500CNY/batch | 5 business days | Custom experiments require a separate quotation |
| Lyophilisation (large scale) | Large lyophiliser capacity is approximately 40 L per run, up to 20,000 vials at 2 mL each. | 10000CNY/batch | 15 business days | Custom experiments require a separate quotation |
| Hydrogel preparation (sodium alginate) | Crosslink sodium alginate with a crosslinker such as calcium ions to form a hydrogel encapsulating EVs. | 2000CNY/batch | 15 business days | Custom experiments require a separate quotation |
| Microneedle preparation (patch) | Combine lyophilised EVs with soluble materials such as hyaluronic acid to prepare dissolvable microneedles for subcutaneous delivery. | 10000CNY/batch | 15 business days | Custom experiments require a separate quotation |
8 services · tax-inclusive reference prices
| Service | Description | Price (tax included) | Turnaround | Notes |
|---|---|---|---|---|
| Functional assay (cell proliferation) | Measure cell proliferation by CCK-8 or EdU; up to 60 wells per batch. | 500CNY/batch | 7 business days | Custom experiments require a separate quotation |
| Functional assay (cell cycle) | Assess cell cycle by flow cytometry using PI staining. | 250CNY/sample | 7 business days | Custom experiments require a separate quotation |
| Functional assay (apoptosis) | Assess apoptosis by Annexin V/PI flow cytometry or TUNEL. | 250CNY/sample | 7 business days | Custom experiments require a separate quotation |
| Functional assay (cell invasion) | Assess cell invasion using Matrigel-coated Transwell inserts. | 250CNY/sample | 7 business days | Custom experiments require a separate quotation |
| Functional assay (cell scratch) | Assess cell migration using a pipette-tip scratch assay. | 250CNY/sample | 7 business days | Custom experiments require a separate quotation |
| Functional assay (cell inflammation) | Induce cellular inflammation pharmacologically, then assess pro- or anti-inflammatory effects of EVs or other agents. | 250CNY/sample | 7 business days | Custom experiments require a separate quotation |
| Functional assay (zebrafish model) | Use zebrafish models to assess EV-related brightening, repair, anti-inflammatory activity and toxicity. | CNY 5,000/10 animals | 20 business days | Custom experiments require a separate quotation |
| Functional assay (mouse model) | Use mouse models to assess EV-related brightening, repair, anti-inflammatory activity and toxicity. | CNY 1,000/animal | 20 business days | Custom experiments require a separate quotation |
For complex samples, non-standard workflows, multi-omics or custom experiments, a project assessment is recommended.
Enquiries: +86 592 5792117 · +86 18965129960 (also WeChat)
Prices shown are tax-inclusive references; actual services, sample requirements and delivery times are governed by the confirmed project order.
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