Service no. | Service scope | Service description | Reference fee | Turnaround |
1 | External loading (transfection) | Use cationic materials to promote interaction with the lipid membrane and subsequent internalization to load nucleic acids into EVs. | 1000 CNY/sample | 3 business days |
2 | External loading (sonication) | Use a probe sonicator to increase membrane permeability and allow small molecules to diffuse into EVs. | 500CNY/sample | 3 business days |
3 | External loading (lipid anchor) | Anchor DSPE-PEG molecules in the EV membrane to attach linked targeting or functional molecules to the surface. | 500 CNY/sample | 3 business days |
4 | External loading (conjugation) | Use a phage-library-selected peptide that specifically binds CD63 to conjugate targeting or functional molecules to the EV surface. | 500CNY/sample | 3 business days |
5 | Click-chemistry loading | Use click chemistry to conjugate targeting or functional molecules to the EV surface. | 2000 CNY/sample | 10 business days |
6 | Endogenous loading by gene knockdown | Transfect plasmids or transduce parent cells to reduce target nucleic-acid or protein expression, affecting the target molecules in EVs. | 2000CNY/sample | 25 business days |
7 | Endogenous loading by gene overexpression | Transfect plasmids or transduce parent cells to increase target nucleic-acid or protein expression, affecting the target molecules in EVs. | 4000 CNY/sample | 25 business days |
8 | Cargo concentration testing (HPLC) | Assess EV cargo loading by high-performance liquid chromatography (HPLC). | 1000CNY/sample | 10 business days |
9 | Cargo concentration testing (LC-MS) | Assess EV cargo loading by liquid chromatography–mass spectrometry (LC-MS). | 1500 CNY/sample | 10 business days |
EV engineering modifies surface molecules or introduces biomolecules into vesicles to investigate targeted delivery and therapeutic applications. The described service can include EV isolation, modification or engineering, purification and testing.
Lifeint provides EV research and application services from sample preparation, isolation and characterization to omics, functional studies, modification and scale-up. It jointly established a biomedical technology centre with Xiamen University School of Pharmaceutical Sciences, built process-development laboratories to GMP standards, and contributed to an EV expert consensus. Its service records cover more than 300 plant species.
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