Service
Service no. | Service scope | Service description | Reference fee | Turnaround |
1 | Standard ultracentrifugation | EV isolation by standard ultracentrifugation. | 200 CNY/50 mL | 3 business days |
2 | Sucrose-cushion ultracentrifugation | EV isolation by sucrose-cushion ultracentrifugation. | 300CNY/50 mL | 3 business days |
3 | Iodixanol-gradient ultracentrifugation | EV isolation by iodixanol density-gradient ultracentrifugation. | 500 CNY/50 mL | 3 business days |
4 | PEG precipitation | EV isolation by PEG precipitation. | 200CNY/50 mL | 3 business days |
5 | Ultrasound nanofiltration (EXODUS) | EV isolation using an ultrasound nanofiltration chip. | 600 CNY/500 μL | 5 business days |
6 | Membrane-sensing peptide bead capture | EV isolation with MSP membrane-sensing peptide-conjugated magnetic beads. | 200CNY/sample | 3 business days |
7 | Large-volume sample isolation (Ceramic membrane + TFF + SEC) | Clarify supernatant with ceramic membranes, concentrate by tangential flow filtration, purify by size-exclusion chromatography and recover EVs by centrifugation. | 1500 CNY/L | 10 business days |
Method overview
EV isolation options include ultracentrifugation (UC), polymer precipitation, tangential flow filtration (TFF), ultrafiltration (UF), size-exclusion chromatography (SEC) and affinity capture. Select a workflow according to sample type, volume, target fraction and downstream experiment, balancing recovery, purity and cost.
The laboratory has ultracentrifuges, automated EV isolation instruments, TFF systems and SEC columns. Existing service configurations cover supernatant inputs from 100 µL to 100 L, subject to sample and workflow assessment. Downstream options include characterization, purity and contaminant checks, and lyophilization.
1. Ultracentrifugation（UC）
Differential ultracentrifugation removes cells, debris and larger particles before enriching EVs, with washing as required. Sucrose cushions or iodixanol gradients can add separation steps to reduce some co-isolated components. Purity and recovery depend on the sample and protocol and must be assessed by characterization and contaminant checks.
2. TFF concentration and SEC purification
TFF directs flow along a membrane for EV concentration and buffer exchange. SEC separates particles by access to pores in its packing material: larger EVs generally elute earlier, while smaller soluble proteins elute later. TFF and SEC can be combined for larger inputs; recovery, purity, processing time and cost require sample-specific evaluation.
3. Automated EV isolation (EXODUS)
EXODUS combines negative-pressure oscillation with double-coupled harmonic oscillation to drive membrane vibration. A nanoporous membrane enriches EVs while some permeable components pass through. The technology was reported in Nature Methods; recovery, integrity and residual contamination still require measurement for each sample type.
4. Automated EV Membrane-sensing peptide capture (EXO-Smart)
Membrane-sensing peptides interact with EV membranes through features such as curvature, lipid composition and lipid-packing defects. Selected peptides can be coupled to magnetic beads for automated capture and elution. The recovered subpopulations and efficiency depend on peptide design and sample conditions and should be evaluated with controls and characterization.
MISEV2023 · EXODUS · Nature Methods · Membrane-sensing peptides
Lifeint provides EV research and application services from sample preparation, isolation and characterization to omics, functional studies, modification and scale-up. It jointly established a biomedical technology centre with Xiamen University School of Pharmaceutical Sciences, built process-development laboratories to GMP standards, and contributed to an EV expert consensus. Its service records cover more than 300 plant species.
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