Service no. | Service scope | Service description | Reference fee | Turnaround |
1 | Animal tissue enzymatic digestion | Prepare tissue supernatant using enzymes such as trypsin and collagenase for subsequent EV isolation. | 200 CNY/sample | 3 business days |
2 | Tissue culture (Transwell) | Collect supernatant from tissue explants cultured in Transwell inserts for subsequent EV isolation. | 10CNY/mL | 3 business days |
3 | Plant tissue enzymatic digestion | Use cellulase and pectinase digestion to prepare plant tissue supernatant for EV isolation. | 200 CNY/sample | 3 business days |
4 | Cell culture (dishes) | Collect conditioned medium using culture dishes or cell factories. | 5CNY/mL | 10 business days |
5 | Cell culture (microcarriers) | Collect supernatant from 3D microcarrier suspension culture. | 2000 CNY/L | 20 business days |
6 | Microbial culture (shake flask) | Collect supernatant from shake-flask cultures. | 2CNY/mL | 5 business days |
Extracellular vesicles (EVs) are nanoscale particles produced by cells that mediate short- and long-range communication and participate in physiological and pathological processes. The source describes a size-based classification referencing MISEV 2018: vesicles at or below 200 nm as small EVs, often called exosomes, and those above 200 nm as medium or large EVs.
Plant, bacterial and mammalian cells can produce EVs containing proteins, nucleic acids, lipids and small-molecule metabolites. EVs are commonly isolated from supernatants obtained through cell culture, tissue digestion or body-fluid processing. The company describes customized upstream culture and sample-preparation options, including tissue-specific digestion or juicing and pretreatment of viscous or mixed solid–liquid samples.
1. Culture dishes
Cell-culture supernatant is a common source for EV isolation and purification. Sample origin, culture duration and conditions can affect EV content and quality, so the source recommends standardizing these factors and selecting cell types appropriate to the experimental objective.
2. Microcarrier culture
The source describes 3D TableTrix® as porous elastic microcarriers with porosity above 90%, controllable particle size of 50–500 μm and uniformity ≤100 μm. It reports cell recovery by specific lysis, residual and safety assessment reports, two CDE pharmaceutical-excipient qualifications and one FDA-DMF pharmaceutical-excipient qualification, and suitability for scale-up of many adherent cells and recovery of cells, viruses and cell products.
3. Tissue digestion
Enzymatic tissue digestion can be used in EV preparation: tissue is digested to a cell suspension and, after cells and debris are removed, EVs are isolated from the supernatant. The source lists animal tissues such as brain, fat, skin, liver and bone, and enzymes such as trypsin, protease, collagenase and dissociation enzymes. Plant roots, stems, leaves, flowers, fruits and seeds can also be processed with enzymes such as pectinase and cellulase.
Lifeint provides EV research and application services from sample preparation, isolation and characterization to omics, functional studies, modification and scale-up. It jointly established a biomedical technology centre with Xiamen University School of Pharmaceutical Sciences, built process-development laboratories to GMP standards, and contributed to an EV expert consensus. Its service records cover more than 300 plant species.
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